For separating the non-volatile mixtures, a special chromatography technique is used, i.e. Thin Layer chromatography. It works on the principle of separation in which the different components are adsorbed on the surface of adsorbent such as silica gel, alumina or cellulose.Usually, silica gel is used as an adsorbent with the size ranging from 400 to 800 meshes for tlc different binding grades including silica gel g, silica gel f, silica gel gf 254, silica gel hf 254. It can be used with or without binder.
This chromatography technique performs several tasks that help in carrying out the separation process with highly positive outcome.
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